simple and provides both high yields and activity of labeled tubulin. Centrifuge the MTP solution at 18,000 r.p.m. in a Sorvall SS-34 rotor at 37 °C for 20 min or in a Beckman TLA 100.3 rotor at ...
TEDC1 and TEDC2, functions in generating centriolar triplet microtubules, and that this is crucial for the proper formation of centriolar subdomains and the stability of centrioles throughout the cell ...
Proteins containing disordered domains perform many important cellular functions. My lab currently has two main projects, one to dissect the role of tubulin's C-terminal tail in microtubule structure ...