The resulting experimental mouse is hemizygous for Cre and homozygous for loxP (D). This is the necessary genotype required to conditionally knock out the target gene in the specific tissue. Laura is ...

All our multiexpression systems (currently MultiBac, MultiMam and MultiColi) rely on in vitro Cre-loxP recombination of Acceptor and Donor plasmids to assemble multiprotein expression vectors. A ...

With the introduction of sequence-specific recombination systems such as FLP/FRT or Cre/LoxP, not only can one dramatically increase the efficiency of generating mosaic animals, but also control ...

In addition, loxP-flanked target genes can differ dramatically with respect to their sensitivity to Cre-mediated recombination. Thus, to be sure about the specificity of a given conditional ...

While this shuffling, known as recombination, is essential for genetic diversity, errors in the process can lead to serious reproductive issues. These failures can result in genetic errors that ...