Flow cytometry may be used to analyse cell cycle in live eGFP Gli2 transduced human keratinocytes, NTERTs cells by loading cells with Hoechst 33342 at 10 ug/ml for 45 mins at 37C. The determination of ...

The Flow Cytometry and Cell Separation Facility provides advanced cell sorting and cell analysis techniques including immunofluorescence detection of 18 colors, cell-cycle distribution, apoptosis ...

flow cytometry and laser-scanning cytometry. The ability to identify cell cycle position through DNA content analysis is fundamental to investigations of cell cycle–regulated protein expression ...

cell cycle analysis, kinetics, proliferation, calibration and statistical comparisons. Other examples include BD CellQuest, a popular commercial flow cytometry data acquisition and analysis ...

Incubate the cells with fixation buffer for 15 to 30 min at 4°C. (Cell aggregation can be avoided by vortexing prior to the addition of the fixation buffer.) Fixed cells should be washed and suspended ...

View Full Profile. Learn about our Editorial Policies. “All of the methodology that existed before flow cytometry suddenly could be applied to the single cell,” said Thomas Jovin, a biophysicist at ...

The Cytometry and Cell Sorting Core at Baylor College of Medicine is heavily subsidized by the College and by several NIH-funded Centers. It is an NIH requirement that any publication using equipment ...

The EC800 flow cytometry analyzer offers you greater versatility ... fluorescent proteins and cell cycle analysis. With up to four lasers and user-configurable fluorescence detectors, the EC800 ...

The management of breast cancer involves various therapeutic approaches. However, many current anticancer treatments indiscriminately damage both cancerous and healthy cells. In cancer treatment, ...

Flow cytometry showed a dose-dependent induction of apoptosis and cell cycle arrest at the sub-G 1 and S phases. Furthermore, OR3 completely inhibited MDA-MB-231 cell migration and demonstrated ...

Pulse processing Muse doublet discrimination This technique is based on the premise that cells in G0 or G1 phases of the cell cycle possess a normal diploid chromosomal, and hence DNA content (2n) ...