However, such experiments were problematic for flow cytometry as there weren’t enough different fluorophores available to identify multiple cell phenotypes at the time. Researchers turned their ...

Methotrexate does not produce a response if it doesn’t eliminate enough malignant B-cells. Flow cytometry can count these cells. If the technology sees that the methotrexate didn’t adequately ...

Pellet 106 cells by centrifugation (250 - 300 x g) and carefully remove supernatant Make up 1X of fixation buffer by adding 5 ml of Cytofix (BD554655) to 10 ml of DPBS Add either 300μl (for microwell ...