What is the Diffraction Limit? The diffraction limit is a fundamental barrier in optical microscopy that sets the minimum size of features that can be resolved using conventional light microscopes. It ...

In 1873, the German physicist Ernst Abbe realized that the resolution of optical imaging instruments, including telescopes and microscopes, is fundamentally limited by the diffraction of light.

Today's super-resolution microscopes have made it possible to observe the nanoscale world with unprecedented detail. However, ...

(Credit: Wyss Institute at Harvard University) The diffraction limit is a fundamental barrier in conventional light microscopy, restricting the spatial resolution to about half the wavelength of the ...

Super-resolution microscopy includes a variety of microscopy techniques that increase the resolving ability of a light microscope well beyond the classical limits dictated by the diffraction barrier.

light sheet microscopy captures dynamic processes in live specimens and intricate structures in cleared samples. Super-Resolution Imaging and Single-Molecule Tracking The MinFlux system enables ...

Dark-field microscopy is a powerful technique used to visualize unstained samples by illuminating them with light at oblique ... microscopy is limited by the diffraction barrier and often requires ...

Raman microscopy combines optical microscopy with ... from which Raman shifted light is collected via the wavelength selective diffraction grating and holographic filtering to remove Rayleigh ...

A quantum cascade laser (QCL) excites molecular vibrations while a spatial light modulator ... within sub-diffraction aggregates, which was impossible with standard fluorescence microscopy.