H3 histone Flow cytometry may be used to analyse cell cycle with propidium iodide (PI) giving peaks of G1 and G2m with S phase located between these peaks. Labelling histone H3 with a fluorescently ...

Hoechst 33342 is one of the few live-cell DNA stains used in cell cycle analysis. However, this dye requires UV-light excitation, and many laboratory flow ... Propidium iodide staining of DNA ...

Harvest cells, want good single cell suspension Count cells Dispense aliquots of 1 million cells into labeled tubes Add 5 ml PBS and centrifuge for 5 min at 400 x g. Re-suspend cell pellet in 300 ul ...

One important aspect of DNA analysis is the ability of the cytometer to exclude cell doublets. In a cytometer where the beam diameter is larger than the nuclear size, we can look at pulse width versus ...