The amount of input target protein sample to use depends on the detection limit of the assay, but 10% of the amount added to each binding reaction (3 μl) is usually sufficient. The unbound target ...

More information: Kejia Li et al, A peptide-centric local stability assay enables proteome-scale identification of the protein targets and binding regions of diverse ligands, Nature Methods (2024).

We often take facts we learn — like the fact that transcription factors bind to DNA — for granted. Or maybe not. See how researchers used DNA footprinting and gel shift assays to prove this idea.

The discovery of a family with sequence similarity 102 member A (Fam102a) protein as a novel bone remodeling factor that regulates both osteoclast and osteoblast differentiation can aid the ...