1 Denaturation: Double-stranded DNA separates into single strands. Annealing ... Several components are involved in a PCR including template DNA, forward and reverse primers, DNA polymerase enzyme, ...

In particular, real-time reverse transcription (RT)-PCR ... uses the thermostable Taq DNA polymerase to extend short single-stranded synthetic primers using the target DNA or cDNA as a template ...

A DNA primer is a single-stranded piece of DNA of known sequence, generally about 20 bases long, that will initiate the building of a new DNA strand that is complementary to the cloned DNA.