and then the temperature ramps back up to 72°C for elongation with thermostable DNA polymerase. PID loops keep the reaction temperature precisely controlled. The whole thing is, as the name ...

Henry Erlich, Children’s Hospital Oakland Research Institute Still dedicated to making the technique work, Cetus Corporation scientists David Gelfand and Susanne Stoffel purified a thermostable DNA ...

6 A combination of highly processive thermostable DNA polymerase and a high-fidelity polymerase with 3′-5′ exonuclease activity helps achieve long-range PCR. 7 This allows the amplification of long ...

8| Extract the nucleic acids with 150 μl of chloroform. Separate the amplified DNA from the residual thermostable DNA polymerase and dNTPs. The DNA can now be ligated to a blunt-ended vector or a ...