The authors' demonstration that impaired olfactory perception reduces caterpillar performance and increases susceptibility to parasitism is solid. These findings highlight the ecological significance ...
We lysed cells in IPH buffer (1 ml; 50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 5 mM EDTA, 0.5% NP-40, 0.1 mM PMSF) at 4 °C for 30 min and removed debris by centrifugation. The cleared lysate was then ...