Two-dimensional polyacrylamide gel electrophoresis (PAGE ... focusing gel composed of a low percentage (2.7%) of acrylamide and containing 9.5 M urea and 2% Nonidet P-40 to maintain protein ...

1| Prepare a 40-ml 4.5% native acrylamide gel (using 1 to 1.5-mm spacers): Prerun the 'gel for 2 h at 10 mA. Large protein complexes greater than 1 MDa in mass are difficult to resolve and migrate ...

Because of the relatively low cost of the ingredients, homemade acrylamide gels are the most economical choice. Scientists can generate numerous hand-cast gels for the same price as a single precast ...