With the dye, cells may be analyzed by fluorescence microscopy, flow cytometry and laser-scanning cytometry. The ability to identify cell cycle position through DNA content analysis is fundamental ...
Flow cytometry may be used to analyse cell cycle in live eGFP Gli2 transduced human keratinocytes, NTERTs cells by loading cells with Hoechst 33342 at 10 ug/ml for 45 mins at 37C. The determination of ...
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