The introduction of green fluorescent protein (GFP) and two-photon microscopy has allowed systematic imaging studies of protein localization in living cells and of the structure and function of ...

It surpasses the diffraction limit of conventional light microscopy by utilizing the properties of fluorescent proteins or dyes that can be photoactivated or photoswitched. PALM enables the ...

Often, even the properties of a protein change when it changes its location—control over its localization ... them under the microscope, they are often linked to a fluorescent, brightly shining ...