Open-Source package for segmentation and analysis of gel electrophoresis images using machine learning. Both a python framework for training/running models and a QuPath GUI extension are available.
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Quality was assessed using 1 mg of DNA visualised after running in a 1% agarose gel. A QuantStudio-7 Real-Time PCR instrument (Thermo Fisher Scientific, USA) and CFX96 Real-Time PCR Detection System ...
Once the PCR reaction was completed, the 10 and 50 ng/μl samples showed detectable bands in gel electrophoresis, but the 1 ng/μl sample did not. Thus, the minimum concentration and amount of template ...