Sense and antisense RNA probes were synthesized using MEGAscript SP6 or T7 kits (Invitrogen) and digoxigenin (DIG)-labeled rNTPs (Roche) according to the manufacturer’s protocol and purified using RNA ...

Another kind of RNA therapy that has been approved by regulators in the U.S. and Europe alike, are antisense oligonucleotides. These work by binding to specific mRNA sequences in order to modify gene ...

During the peer-review process the editor and reviewers write an eLife assessment that summarises the significance of the findings reported in the article (on a scale ranging from landmark to useful) ...

New research published in the Journal of Chromatography A examines the effect of flow rate on plate height and resolution for antisense oligonucleotides ... ONs are short single- or double-stranded ...

Now, researchers used cryoelectron microscopy (cryo-EM) to elucidate the structure of the Nipah virus polymerase complex ... for both transcription and RNA replication and mutations that have ...

Looking for lightning-fast portable storage at a killer price? Samsung’s T7 Portable SSD with 2TB capacity is up for a fantastic discount that makes this speed demon more accessible than ever.

Furthermore, the T7 is backed up by a five-year/124,000-mile (200,000-kilometer) warranty. The headlight numbers for the all-new Transporter are up to 6.8 cubic meters (240.1 cubic feet ...

Antisense cRNA was synthesized and amplified by in vitro transcription of the second-strand cDNA template using T7 RNA polymerase. cRNA was purified using Nucleic Acid Binding Beads, washed twice with ...

For antisense riboprobe synthesis, the mVGLUT1-pTOPO and mVGLUT2-pTOPO plasmids were linearized with HindIII and BamHI, respectively, and transcribed with T7 RNA polymerase (Promega, Madison, WI, USA) ...