The EMBO Journal3d
Exonuclease action of replicative polymerase gamma drives damage-induced mitochondrial DNA clearance
How cells respond to mitochondrial DNA (mtDNA) damage is not fully understood. This study uncovers a mechanism of mtDNA ...
autoevolution18d
2025 Volkswagen Transporter T7 Now Available to Order in the United Kingdom
Furthermore, the T7 is backed up by a five-year/124,000-mile (200,000-kilometer) warranty. The headlight numbers for the all-new Transporter are up to 6.8 cubic meters (240.1 cubic feet ...
eLife18d
Multiplexed Assays of Human Disease-relevant Mutations Reveal UTR Dinucleotide Composition as a Major Determinant of RNA Stability
This valuable study combines massively parallel reporter assays and regression analysis ... to in vitro transcription using MEGAscript™ T7 Transcription Kit (Thermo Fisher Scientific). To cap the RNA ...
eLife18d
Multiplexed Assays of Human Disease-relevant Mutations Reveal UTR Dinucleotide Composition as a Major Determinant of RNA Stability
Taken together, this study will be of interest to researchers broadly studying post-transcriptional gene regulation and also to scientists using massively parallel reporter assays. UTRs contain ...
Frontiers27d
Coexisting patterns and significance of serum HBV RNA and HBV DNA in patients with treatment-naïve chronic hepatitis B virus infection
The HBV RNA levels were determined using a simultaneous amplification and testing assay. Conclusion ... templates and/or insufficient RNA-dependent DNA polymerase (RDDP) reverse transcriptase (RT) ...
C&EN1mon
RNA Translocation through Protein Nanopores: Interlude of the Molten RNA Globule
Direct translocation of RNA with secondary structures using single-molecule electrophoresis through protein nanopores shows significant fluctuations in the measured ionic current, in contrast to ...
C&EN1mon
Microarray-Based Methodology for Lipid Profiling, Enzymatic Activity, And Binding Assays in Printed Lipid Raft Membranes from Astrocytes and Neurons
To study differences in lipid profiles between raft domains, the MALDI-MS assay was performed on RMMAs. To evaluate the preservation of native protein activities (enzymatic activity and ligand binding ...
Frontiers1y
A self-amplifying RNA vaccine provides protection in a murine model of bubonic plague
Messenger RNA (mRNA) vaccine manufacture offers a more streamlined approach ... together with the most immunogenic F1 construct (hereafter denoted F1). Figure 2. Assay of NAb directed toward the V ...