C&EN15d
Exonuclease-III Assisted Signal Cycle Integrating with Self-Priming Mediated Chain Extension for Sensitive and Reliable MicroRNA Detection
Department of Vascular Surgery, The Dingli Clinical Institute of Wenzhou Medical University (Wenzhou Central Hospital), Wenzhou City, Zhejiang Province 325000, China Laboratory of Wenzhou Pan-Vascular ...
Frontiers20d
MicroRNA Signature of Traumatic Brain Injury: From the Biomarker Discovery to the Point-of-Care
cDNA products are then amplified using conventional TaqMan PCR. 2) The addition of a poly(a ... (143) developed a protocol using probe:miRNA duplex binded to p19-functionalized magnetic beads, which ...
BMJ20d
Circulating miR-15b and miR-130b in serum as potential markers for detecting hepatocellular carcinoma: a retrospective cohort study
Objective Serum α-fetoprotein (AFP) is the most commonly used biomarker for screening hepatocellular carcinoma (HCC) but fails to detect about half of the patients. Thus, we investigated if ...
gut.bmj20d
MicroRNA-221/222 upregulation indicates the activation of stellate cells and the progression of liver fibrosis
The GAPDH level was used to normalise the relative abundance of mRNAs. To detect miRNA expression, the RT reaction was performed using the TaqMan MicroRNA Assay (Applied Biosystems). Primers for PCR ...
technologynetworks1mon
Choose the Right Reaction Chemistry for Real-Time PCR
TaqMan assays and SYBR Green dye are two popular approaches, each offering distinct advantages depending on a lab’s needs. By understanding these distinctions, researchers can select the approach that ...
C&EN1mon
Unraveling the Impact of Polyethylenimine-Coated Gold Nanoparticle Size on the Efficiency of Sandwich-Style Electrochemical Immunosensors
Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand Center of Excellence for Innovation in Chemistry, Chiang Mai University, Chiang Mai 50200, Thailand ...
eLife2y
Feedback between a retinoid-related nuclear receptor and the let-7 microRNAs controls the pace and number of molting cycles in C. elegans
The manuscript nicely advances our understanding of the roles of heterochronic genes and the NHR-23 nuclear receptor transcription factor in the regulation of the temporal dynamics of molting behavior ...
Frontiers2y
MicroRNA Expression in Circulating Leukocytes and Bioinformatic Analysis of Patients With Moyamoya Disease
Also, the differentially expressed miRNAs should not have probes with flag A in at least one group ... A total of 100 ng of purified RNA was reversely transcribed to cDNA using the Taqman MicroRNA ...
eLife5y
A regulatory microRNA network controls endothelial cell phenotypic switch during sprouting angiogenesis
We described a microRNA network which contributes to the observed down- and upregulation of proliferative and migratory genes, respectively. Global analysis of miRNA–target gene interactions ...
Nature11y
MicroRNA-206 is involved in hypoxia-induced pulmonary hypertension through targeting of the HIF-1α/Fhl-1 pathway
Total RNA was extracted and cDNA was generated by reverse transcription using the miRVanaâ„¢ PARISâ„¢ Kit (Ambion, Austin, TX, USA) and the TaqMan MiR Reverse Transcription Kit (Applied Biosystems ...