Leveraging this information, we developed a TaqMan probe-based quantitative real-time PCR (qPCR) assay that is uniquely tailored to detect both PEDV GI and GII subtypes. Results: Additionally, this ...

by quantitative methylation-specific PCR using the methylation-specific TaqMan probe demonstrated 87% sensitivity [114/141; 95% confidence interval (CI), 80.0% to 92.3%] and 95.2% specificity (10/125; ...

qPCR reactions were performed in duplicate, in scaled-down 10 μL reaction volumes containing 5 μl TaqMan 2X Universal PCR Master Mix with No AmpErase UNG (Applied Biosystems), 0.5 μl 20X ...

Real-time PCR (qPCR) is the gold-standard for sensitive, specific detection and quantification of nucleic acid targets through fluorescence-based techniques. TaqMan assays and SYBR Green dye are two ...

The GoTaq ® Probe 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase and ...

This is an important and solid study that examines the role of TFAM, a protein that helps maintain mtDNA, in mtDNA mutator mice. The authors have demonstrated that TFAM's counteractive role in mtDNA ...

The findings emerged from an initial crash investigation report released Monday local time by South Korea’s transport ministry, which oversees the probe with the U.S., Boeing and the jet’s ...

PARIS, Jan 28 (Reuters) - French investigators said on Tuesday they had opened a judicial probe into money laundering, tax fraud and other charges at Binance, the world's largest cryptocurrency ...

French investigators have opened a probe into social media platform X over allegations that the company owned by billionaire Elon Musk manipulated its algorithm, the Paris public prosecutor's office ...

This important study focused on characterizing clonally derived MSC populations from the synovium of normal and osteoarthritis (OA) patients, demonstrating their potential to regenerate cartilage in ...

The Food and Drug Administration, a federal agency of the HHS, usually releases updates on probes of outbreaks linked to contaminated food every Wednesday, but the agency did not release them this ...