The DeltaVision OMX V4 enables Total Internal Reflection Fluorescence Microscopy (TIRF ... when the incident light is totally reflected at the interface between the glass coverslip and the specimen.

and the ray changes from just refracting to total internal reflection, is called the critical angle. The diagram below shows the light refracting from glass into air. For light travelling from ...

The process happening to the light at Y is total internal reflection. (a ... of light increases as it passes from a dense medium (glass) into a less dense medium (air) so, as light passes point ...

The Wolfson Bioimaging Facility provides total internal reflection fluorescence microscopy (TIRF ... an evanescent field (caused when the illuminating laser reflects off the glass/water or ...