Two-dimensional polyacrylamide gel electrophoresis (PAGE ... focusing gel composed of a low percentage (2.7%) of acrylamide and containing 9.5 M urea and 2% Nonidet P-40 to maintain protein ...

Because of the relatively low cost of the ingredients, homemade acrylamide gels are the most economical choice. Scientists can generate numerous hand-cast gels for the same price as a single precast ...

1| Prepare a 40-ml 4.5% native acrylamide gel (using 1 to 1.5-mm spacers): Prerun the 'gel for 2 h at 10 mA. Large protein complexes greater than 1 MDa in mass are difficult to resolve and migrate ...