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They were particularly drawn to features of T7 DNA polymerase—derived from the T7 bacteriophage that infects Escherichia coli—due to its ability to synthesize long stretches of DNA by binding and ...
This historical perspective integrates 50 years of research on SOS mutagenesis in Escherichia coli with the proverbial ... recombination—that feature DNA polymerase V. Genetic and biochemical ...
As DNA polymerase makes its way down the unwound ... In the prokaryotic bacterium E. coli, replication can occur at a rate of 1,000 nucleotides per second. In comparison, eukaryotic human DNA ...
Erlich led the team of scientists at the Cetus Corporation that developed the PCR method. In the 1950s, scientists discovered Escherichia coli DNA polymerase and immediately put it to use synthesizing ...
More information: Sofya K Garushyants et al, Mutational Signatures in Wild Type Escherichia coli Strains Reveal Predominance ...
Conventionally, testing for fecal contamination involves detecting fecal indicator bacteria (FIB), such as E. coli ... method uses polymerase chain reaction (PCR) to detect CLP DNA, making ...