Endpoint analysis: Scientists analyze PCR products typically using agarose gel electrophoresis. Real-time PCR detects and quantifies DNA as the reaction progresses. This quantifies the specific amount ...

their gel electrophoresis results were also large smears. They needed to determine if their product was part of this smear. Over time, the team experienced a series of minor celebrations as they ...

Analyze the PCR product by agarose gel electrophoresis, and quantify the DNA yield using the PicoGreen dsDNA kit. The amount of DNA used in the emulsion PCR can vary over a relatively wide range.

At the completion of the reaction, amplification products are analyzed by size fractionation using gel electrophoresis. Real-time PCR follows the same course, except that the products are detected ...