The total reaction volume was 50 μL, containing 1× Transcription buffer, 100 U of T7 RNA polymerase, 0.5 mM NTPs, 15 µL of the annealed product of HybProbe template and T7F ... signal reporter ...

These merged fragments were subsequently cloned into the backbone that had been digested with XbaI, which contained the T7 RNA polymerase promoter and terminator. After that, the RSV pre - F antigen, ...

Attribution (BY): Credit must be given to the creator. The study of “resource allocator” bacteriophage T7 RNA polymerase (T7RNAP) has garnered significant interest, particularly for optimizing ...

Christian Doppler Laboratory for production of next-level biopharmaceuticals in E. coli, BOKU University, Department of Biotechnology and Food Science, Vienna 1190, Austria ...