were utilized as templates for RNA in vitro transcription with T7 RNA polymerase (Vazyme), following manufacturer’s protocol. The reaction was then incubated at 37 °C for 16 hours, followed by DNase I ...

were utilized as templates for RNA in vitro transcription with T7 RNA polymerase (Vazyme). The reaction was then incubated at 37 °C for 16 hours, followed by DNase I treatment for 30 minutes at 37 °C.

In frame translation initiation sites within coding sequences drive the expression of N-terminally truncated proteoforms.

Here, the authors show that under acute heat shock, it selectively arrests cellular transcription and translation, independent of the signal peptide. Wang et al. demonstrate that the vascular ...

Yeah, so in talking with more than a dozen engineers and academic researchers, my co-reporter Hilka Shellman and I found that this particular AI powered transcription tool makes things up that can ...

Whilst the ultimate outcome of the lytic cycle is production of new phage progeny and death of the host bacterial cell, this is a multistep process involving precise coordination of gene transcription ...

In the same way, the K-tech T7 is still unmistakably a Ténéré - it’s just that bit better. Just like with the film, the difference is more pronounced when you go back to the original version ...

related to DHAV-1 RNA translation) was established with a ScriptMAX Thermo T7 Transcription Kit. Next, 3 μl of DNAzyme (2 pmol/ml) and 3 μl of substrate RNA (2 pmol/ml) were added to buffer with 50 ...

RNA Interference (RNAi) triggered by dsRNA has evolved as a promising strategy to control insects in a species-specific manner. In this context, we review the methods for mass production of dsRNA, the ...

You’ve certainly heard about antibodies, peptides, and even DNA as therapeutics, but what about RNA? In this review, we take a look at what’s happening in a field that some believe may be as big a ...

Please view our affiliate disclosure. For many marketers, researchers, and organizations who spend hours manually transcribing interviews, podcasts, and meetings, finding an efficient and reliable ...