cDNA products are then amplified using conventional TaqMan PCR. 2) The addition of a poly(a ... (143) developed a protocol using probe:miRNA duplex binded to p19-functionalized magnetic beads, which ...

Objective Serum α-fetoprotein (AFP) is the most commonly used biomarker for screening hepatocellular carcinoma (HCC) but fails to detect about half of the patients. Thus, we investigated if ...

The GAPDH level was used to normalise the relative abundance of mRNAs. To detect miRNA expression, the RT reaction was performed using the TaqMan MicroRNA Assay (Applied Biosystems). Primers for PCR ...

TaqMan assays and SYBR Green dye are two popular approaches, each offering distinct advantages depending on a lab’s needs. By understanding these distinctions, researchers can select the approach that ...

Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand Center of Excellence for Innovation in Chemistry, Chiang Mai University, Chiang Mai 50200, Thailand ...

Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua ...

To assay expression of miRNA, TaqMan Advanced miRNA Assays (ThermoFisher A25576) were used with probes against hsa-miR-361–5p as a housekeeping control and hsa-miR-4707–3p (Assay ID: 478056 and 479946 ...

Also, the differentially expressed miRNAs should not have probes with flag A in at least one group ... A total of 100 ng of purified RNA was reversely transcribed to cDNA using the Taqman MicroRNA ...

We described a microRNA network which contributes to the observed down- and upregulation of proliferative and migratory genes, respectively. Global analysis of miRNA–target gene interactions ...