by quantitative methylation-specific PCR using the methylation-specific TaqMan probe demonstrated 87% sensitivity [114/141; 95% confidence interval (CI), 80.0% to 92.3%] and 95.2% specificity (10/125; ...

qPCR reactions were performed in duplicate, in scaled-down 10 μL reaction volumes containing 5 μl TaqMan 2X Universal PCR Master Mix with No AmpErase UNG (Applied Biosystems), 0.5 μl 20X ...

For the TaqMan qPCR reactions,10 µL consisted of Premix Ex Taq (2X), 0.4 µL of each primer (10 mM), 0.8 µL probe, 0.2 µL ROX Reference Dye (50X), 2 µL of template DNA, and double-distilled water (ddH ...

Each one-step RT-qPCR reaction contained 10 µL of extracted RNA, 4 µL TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific, Massachusetts, USA) in a final reaction volume of 20 µL. Primer and ...

The GoTaq ® Probe 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase and ...

2012;12(7):731-754. SYBR Green and dual-hybridization probes are often used for melting point analysis at the end of the amplification. qPCR presents a number of advantages over conventional PCR ...

This is an important and solid study that examines the role of TFAM, a protein that helps maintain mtDNA, in mtDNA mutator mice. The authors have demonstrated that TFAM's counteractive role in mtDNA ...

The findings emerged from an initial crash investigation report released Monday local time by South Korea’s transport ministry, which oversees the probe with the U.S., Boeing and the jet’s ...

Lunar Trailblazer is part of a growing list of probes and landers heading toward the moon to search for water ice. LITTLETON, Colorado — A university-led lunar orbiter designed to pinpoint the ...

Here’s how it works. Astronomers are trying to understand a mysterious cosmic explosion that the Einstein Probe spacecraft spotted in April last year. The highly energetic explosion was ...

Three House panels – committees on public order, public information, and information and communications technology – will jointly conduct the probe, which Sta. Rosa City, Laguna Rep.