This protocol describes the first stages of Southern blotting: digestion of genomic DNA with restriction enzymes, separation of the resulting fragments by gel electrophoresis, and capillary ...
In this form of pulsed-field gel electrophoresis (transverse ... 9 | Transfer the DNA directly to a nylon membrane by capillary blotting in denaturation solution (1.5 M NaCl, 0.5 M NaOH).
Back then, Applied Biosystems' first automated DNA sequencer ... is a capillary electrophoresis-based affair. Each capillary is the equivalent of one slab gel lane, and the 3730xl can run 96 of them ...
and does not require post-electrophoresis staining for fast and convenient results. Both the DNA and RNA systems are also suitable for downstream applications such as qPCR or sequencing. For DNA ...
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