Jul 25, 2023 · polymerase possesses a specific type of 5´- 3´ exonuclease activity called 5´ flap endonuclease activity, which catalyzes the cleavage of 5´ DNA flaps from a DNA duplex that …

Exonucleases can act as proofreaders during DNA polymerisation in DNA replication, to remove unusual DNA structures that arise from problems with DNA replication fork progression, and …

DNA polymerase I also has 3' to 5' and 5' to 3' exonuclease activity, which is used in editing and proofreading DNA for errors. The 3' to 5' can only remove one mononucleotide at a time, and …

May 1, 2002 · Many polymerases (for instance, pols γ, δ and ɛ) contain another enzymatic activity in the DNA polymerase polypeptide — a 3′–5′ exonuclease. This enzymatic activity can …

Oct 7, 2024 · A well-known enzyme with 5′ to 3′ exonuclease activity is DNA Polymerase I, which plays an integral role in prokaryotic DNA replication. This enzyme removes the RNA primer …

Dec 29, 2002 · On control templates, the 3′→5′ exonuclease activity of T4 DNA polymerase exhibits “proofreading” activity by degrading terminal mispaired substrates (e.g. G·T) at a …

DNA exonucleases, enzymes that hydrolyze phosphodiester bonds in DNA from a free end, play important cellular roles in DNA repair, genetic recombination and mutation avoidance in all …

Replicative DNA polymerases possess 3′ → 5′ exonuclease activity to reduce misincorporation of incorrect nucleotides by proofreading during replication.

May 1, 2015 · Proofreading by the bacteriophage T4 and RB69 DNA polymerases requires a β hairpin structure that resides in the exonuclease domain. Genetic, biochemical and structural …

Dec 29, 2002 · The 3'-->5' exonuclease activity intrinsic to several DNA polymerases plays a primary role in genetic stability; it acts as a first line of defense in correcting DNA polymerase …